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Fluorescence staining using TOTO-1 (514/533; Invitrogen, Carlsbad, CA) was performed to confirm the damage to spore membranes caused by the antifungal substance. forza toto login alternatif The spores were collected as described above and resuspended in 300 µL diluted TOTO-1 (1/5000 with TE buffer) for 3 min. mana toto